About Us

We are a Californian Biotech company setup in 2014 with the aim to offer competitive services to our clients / sponsors. In the process, we have also leveraged our strength in offering cost competitive product solutions for many products through our China counter-part.
We are happy to announce that in August 2015, a startup company based in South California-Krishlife is now part of us.

Our products, covering from genes to proteins, include cDNA libraries, Primers, Proteins and DNA Marker, Natural and Recombinant proteins (prokaryotic, yeast or eukaryotic system), Monoclonal and Polyclonal antibodies, target gene clone kits, ELISA and CLIA kits. Our products involve a variety of areas containing cytokines, signal transduction, CD & adhesion molecules, enzyme & kinase, metabolism, endocrine, reproduction immune, infection immunity, tumor immunity (apoptosis, tumor marker), autoimmunity, immunomodulatory and much more.

Additionally, KINESISDx is well positioned to offer custom-built services to meet our customers' specific needs, such as special target gene clone, target protein expression or detection assay kits, modification of special small molecules, development of biomarkers, comparator studies, etc. To meet the Customer's need is our ultimate aim.

We offer our products and services with Quality Guarantee System. KINESISDx is mainly focused on providing high-quality gene-protein-antibody related research tools and services with perfect technical support security system. Our scientific staff team provides expert technical support to each customer in every step of the service, which can guarantee the benefit of our customers.
We look forward to serving your research needs and becoming your long term research partner.

Our Services

To meet the Customer's need is our ultimate aim.



KINESISDx offers custom-built services to customers' specific needs, such as special target gene clone, target protein expression or detection assay kits, modification of special small molecules, development of biomarkers,comparator studies, etc.

KinesisDx is mainly focused on providing high-quality gene-protein-antibody related research tools and services with perfect technical support security system.
Talk to us for more details.
Email us at : services@kinesisdx.com

Products



We offer our products with Quality Guarantee System.
KINESISDx is mainly focused on providing high-quality gene-protein-antibody
related research tools and services with perfect technical support security system.

Please click below for all our products.

All Resources

(Trouble Shooting Tips)
Having trouble with an experiment? Use our checklists to help identify the problem. We have many years of experience of doing antibody-related experiments and are delighted to pass that knowledge on to you.

ELISA

I. No signal
1. Ensure enough amounts of antigens are fixed to wells.
2. Decrease the milk percentage in blocking buffer and/or antibody dilution buffer as non-fat dry milk may mask some antigen sites.
3. Increase the concentration of primary antibody &/or secondary antibody.
4. Increase the incubation time for primary antibody and/or secondary antibody incubation.
5. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.
6. Is the antibody correctly stored?

II. High background
1. Increase number of washes in steps to avoid insufficient washing.
2. Increase the blocking time and/or adjust milk concentration to encounter the problem of insufficient blocking.
3. Reduce the concentration of primary antibody or secondary antibody.
4. Check buffers for possible bacterial contaminations.

Western Blot

I. No bands observed/ weak bands observed
1. Increase the amount of total protein (or cell lysate) loaded on gel. Total lysate should be loaded at minimum 100 ug per lane, more is better.
2. Try to use PVDF membrane (or PSQ membrane for low molecular weight proteins) rather than nitrocellulose membrane.
3. Ensure a good transfer between membrane and gel.
4. Decrease the milk percentage in blocking buffer and/or antibody dilution buffer as non-fat dry milk may mask some antigen sites.
5. Increase primary and/or secondary antibody concentration.
6. Extend incubation time for either primary antibody or secondary antibody incubation or both.
7. Reduce the number of washes to minimum in steps to encounter the problem of low protein-antibody binding.
8. Are ECL reagents fresh?
9. Confirm the expression of targeted proteins in cells.
10. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.
11. Is the antibody correctly stored?

II. Non-specific bands/ High background
1. Increase the blocking time and/or adjust milk concentration to encounter the problem of insufficient blocking.
2. Reduce the concentration of primary antibody and/or secondary antibody.
3. Reduce the amount of total protein (or cell lysate) loaded on gel.
4. Increase the number of washes in steps to remove some non-specific binding of primary and/or secondary antibodies.
5. Ensure there is neither aggregation of analyte nor degradation of analyte.
6. Check buffers for possible bacterial contaminations.
7. Reduce film exposure time.

Immunohistochemistry

I. No Staining
1. Does your tissue section do present the specific antigen?
2. Deparaffinize sections longer or change fresh xylene solvent as inadequate deparaffinization may be encountered.
3. Increase the concentration of primary and/or secondary antibodies.
4. Increase the incubation time for primary and/or secondary antibodies.
5. Adjust the time of post fixation or try different fixatives by considering improper tissue fixation.
6. Is your antibody properly stored?
7. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.
8. Overstaining/ High background
9. Block endogenous peroxidase activity by 3 % hydrogen peroxide.
10. Increase the blocking time.
11. Reduce the concentration of primary and/or secondary antibodies.
12. Reduce the incubation time for primary and/or secondary antibodies.
13. Increase the number of washes in steps.
14. Avoid samples being dried out.
15. Check buffers for possible bacterial contaminations.

Immunofluorescence

I. No staining/ weak staining
1. Increase the concentration of primary and/or secondary antibodies.
2. Increase the incubation time for primary and/or secondary antibodies.
3. Are proteins expressed in cells?
4. Is your antibody properly stored?
5. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.

II. High background
1. Increase the blocking time.
2. Reduce the concentration of primary and/or secondary antibodies.
3. Reduce the incubation time for primary and/or secondary antibodies.
4. Increase the number of washes in steps.
5. Check buffers for possible bacterial contaminations.

Get in Touch

We are here to solve all your querries related to our products. Reach out to us and we will revert back as soon as we can.
Feel free to Contact Us Anytime