About Us

We are a Californian Biotech company setup in 2014 with the aim to offer competitive services to our clients / sponsors. In the process, we have also leveraged our strength in offering cost competitive product solutions for many products through our China counter-part.
We are happy to announce that in August 2015, a startup company based in South California-Krishlife is now part of us.

Our products, covering from genes to proteins, include cDNA libraries, Primers, Proteins and DNA Marker, Natural and Recombinant proteins (prokaryotic, yeast or eukaryotic system), Monoclonal and Polyclonal antibodies, target gene clone kits, ELISA and CLIA kits. Our products involve a variety of areas containing cytokines, signal transduction, CD & adhesion molecules, enzyme & kinase, metabolism, endocrine, reproduction immune, infection immunity, tumor immunity (apoptosis, tumor marker), autoimmunity, immunomodulatory and much more.

Additionally, KINESISDx is well positioned to offer custom-built services to meet our customers' specific needs, such as special target gene clone, target protein expression or detection assay kits, modification of special small molecules, development of biomarkers, comparator studies, etc. To meet the Customer's need is our ultimate aim.

We offer our products and services with Quality Guarantee System. KINESISDx is mainly focused on providing high-quality gene-protein-antibody related research tools and services with perfect technical support security system. Our scientific staff team provides expert technical support to each customer in every step of the service, which can guarantee the benefit of our customers.
We look forward to serving your research needs and becoming your long term research partner.

Our Services

To meet the Customer's need is our ultimate aim.



KINESISDx offers custom-built services to customers' specific needs, such as special target gene clone, target protein expression or detection assay kits, modification of special small molecules, development of biomarkers,comparator studies, etc.

KinesisDx is mainly focused on providing high-quality gene-protein-antibody related research tools and services with perfect technical support security system.
Talk to us for more details.
Email us at : services@kinesisdx.com

Products



We offer our products with Quality Guarantee System.
KINESISDx is mainly focused on providing high-quality gene-protein-antibody
related research tools and services with perfect technical support security system.

Please click below for Antibodies






Please click below for HPLC Kits



Please click below for Coagulation Assays Kits

All Resources

(Trouble Shooting Tips)
Having trouble with an experiment? Use our checklists to help identify the problem. We have many years of experience of doing antibody-related experiments and are delighted to pass that knowledge on to you.

ELISA

I. No signal
1. Ensure enough amounts of antigens are fixed to wells.
2. Decrease the milk percentage in blocking buffer and/or antibody dilution buffer as non-fat dry milk may mask some antigen sites.
3. Increase the concentration of primary antibody &/or secondary antibody.
4. Increase the incubation time for primary antibody and/or secondary antibody incubation.
5. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.
6. Is the antibody correctly stored?

II. High background
1. Increase number of washes in steps to avoid insufficient washing.
2. Increase the blocking time and/or adjust milk concentration to encounter the problem of insufficient blocking.
3. Reduce the concentration of primary antibody or secondary antibody.
4. Check buffers for possible bacterial contaminations.

Western Blot

I. No bands observed/ weak bands observed
1. Increase the amount of total protein (or cell lysate) loaded on gel. Total lysate should be loaded at minimum 100 ug per lane, more is better.
2. Try to use PVDF membrane (or PSQ membrane for low molecular weight proteins) rather than nitrocellulose membrane.
3. Ensure a good transfer between membrane and gel.
4. Decrease the milk percentage in blocking buffer and/or antibody dilution buffer as non-fat dry milk may mask some antigen sites.
5. Increase primary and/or secondary antibody concentration.
6. Extend incubation time for either primary antibody or secondary antibody incubation or both.
7. Reduce the number of washes to minimum in steps to encounter the problem of low protein-antibody binding.
8. Are ECL reagents fresh?
9. Confirm the expression of targeted proteins in cells.
10. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.
11. Is the antibody correctly stored?

II. Non-specific bands/ High background
1. Increase the blocking time and/or adjust milk concentration to encounter the problem of insufficient blocking.
2. Reduce the concentration of primary antibody and/or secondary antibody.
3. Reduce the amount of total protein (or cell lysate) loaded on gel.
4. Increase the number of washes in steps to remove some non-specific binding of primary and/or secondary antibodies.
5. Ensure there is neither aggregation of analyte nor degradation of analyte.
6. Check buffers for possible bacterial contaminations.
7. Reduce film exposure time.

Immunohistochemistry

I. No Staining
1. Does your tissue section do present the specific antigen?
2. Deparaffinize sections longer or change fresh xylene solvent as inadequate deparaffinization may be encountered.
3. Increase the concentration of primary and/or secondary antibodies.
4. Increase the incubation time for primary and/or secondary antibodies.
5. Adjust the time of post fixation or try different fixatives by considering improper tissue fixation.
6. Is your antibody properly stored?
7. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.
8. Overstaining/ High background
9. Block endogenous peroxidase activity by 3 % hydrogen peroxide.
10. Increase the blocking time.
11. Reduce the concentration of primary and/or secondary antibodies.
12. Reduce the incubation time for primary and/or secondary antibodies.
13. Increase the number of washes in steps.
14. Avoid samples being dried out.
15. Check buffers for possible bacterial contaminations.

Immunofluorescence

I. No staining/ weak staining
1. Increase the concentration of primary and/or secondary antibodies.
2. Increase the incubation time for primary and/or secondary antibodies.
3. Are proteins expressed in cells?
4. Is your antibody properly stored?
5. Confirm host species and Ig type of primary antibody as incorrect secondary antibody may be used.

II. High background
1. Increase the blocking time.
2. Reduce the concentration of primary and/or secondary antibodies.
3. Reduce the incubation time for primary and/or secondary antibodies.
4. Increase the number of washes in steps.
5. Check buffers for possible bacterial contaminations.



Bibliography

Effect of escitalopram and carbidopa on bone markers in Wistar rats: a preliminary experimental study R Wadhwa, M Kumar, YN Paudel, R Iqbal… - Journal of bone and …, 2018 - Springer … The ELISA kit for the quantification of DKK-1 and sclerostin levels in femora bone was obtained from Kinesis DX (Los Angeles) and Sincere Biotech co., Ltd. (China) … The estimation of RANKL was done by ELISA kit obtained from Kinesis DX (Los Angeles) …

Ameliorative effect of beraprost sodium on celecoxib-induced cardiotoxicity in rats (Winter 2018) S Ahmad, BP Panda, M Fahim, N Dhyani… - Iranian Journal of …, 2017 - ijpr.sbmu.ac.ir … Ltd. ELISA Kits for estimations of Troponin-T (Tn-T) and Tumour necrosis factor-alpha (TNF-α) were obtained from Kinesis DX Ltd., USA and Krishgen biosystem Ltd., India respectively … Serum Tn-T and TNF-α was measured by using rat specific Tn-T (Kinesis DX Ltd …

L-cysteine capped lanthanum hydroxide nanostructures for non-invasive detection of oral cancer biomarker S Tiwari, PK Gupta, Y Bagbi, T Sarkar… - Biosensors and …, 2017 - Elsevier … min. This immunosensor was found to be more advanced in terms of high sensitivity and low detection limit as compared to previously reported biosensors and commercially available ELISA kit (Kinesis DX).

Anticancer Activity of Cyclophosphamide Nanoparticles against EACCB Swiss - pdfs.semanticscholar.org … collected and used for cytokine quantification by ELISA for mouse IL-12, IL-6, TNF-alpha, VEGF and MMP-3according to the manufacturer's instructions (Kinesis Dx, USA). Standard … centrifugation at 2000rpm for 10 minutes.Standard curve for TAC from kit (Kinesis Dx …

A biocompatible serine functionalized nanostructured zirconia based biosensing platform for non-invasive oral cancer detection S Kumar, JG Sharma, S Maji, BD Malhotra - RSC Advances, 2016 - pubs.rsc.org … The CYFRA-21-1 and anti-CYFRA-21-1 were obtained from Ray Biotech, Inc., India. These biomolecules were further diluted by using PBS buffer of pH 7.0. CYFRA-21-1 ELISA Kit was purchased from KinesisDX, USA. Instrumentation …

Evaluation of Anti-Inflammatory and Immunomodulatory Effects of Aqueous Extract of Solanum Xanthocarpum in Experimental Models of Bronchial Asthma K Gulati - EC Pharmacology and Toxicology, 2016 - ecronicon.com … Ovalbu- min (OVA) and prednisolone were procured from Sigma-Aldirch, USA. The ELISA assay kits (rat) for TNF-α, IL-4, IL-6, IFN-γ were procured from Diaclone, France, whereas assay kit for ovalbumin specific IgE was procured from KinesisDX, USA …

Nanostructured zirconia decorated reduced graphene oxide based efficient biosensing platform for non-invasive oral cancer detection S Kumar, JG Sharma, S Maji, BD Malhotra - Biosensors and Bioelectronics, 2016 - Elsevier … Biotech, Inc., India. These biomolecules were further diluted by using PBS buffer of pH 7.0. CYFRA-21-1 ELISA Kit was purchased from Kinesis DX, USA. 2.2. Fabrication of biosensing platform. Nanostructured ZrO 2 decorated …

Highly sensitive protein functionalized nanostructured hafnium oxide based biosensing platform for non-invasive oral cancer detection S Kumar, S Kumar, S Tiwari, S Augustine… - Sensors and Actuators B …, 2016 - Elsevier … These biomolecules were further diluted using PBS buffer of pH 7.0. CYFRA-21-1 ELISA Kit was purchased from Kinesis DX, USA. 2.2. Synthesis of hafnia nanoparticles. Low temperature hydrothermal process was used for the synthesis of hafnia nanoparticles …

Neutrophil elastase levels in the gingival crevicular fluid following hyaluronan gel application in the treatment of chronic periodontitis: A randomized split … S Mallikarjun, A Neelakanti, HM Babu, SB Pai… - Indian Journal of Dental …, 2016 - ijdr.in … Biochemical analysis of gingival crevicular fluid samples The test kit employed for this study includes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) test kit (Human NE ELISA, KINESIS Dx, Los Angeles, CA, USA) [Figure 5] to determine the levels of …

Aegle marmelos differentially affects hepatic markers of glycolysis, insulin signalling pathway, hypoxia, and inflammation in HepG2 cells grown in fructose … H Aggarwal, J Nair, P Sharma, R Sehgal… - Molecular and cellular …, 2018 - Springer … Biochemical estimations. Biochemical estimation of Phosphofructokinase (PFK) (Kinesis DX), PI3K (Bioassay Laboratory Technology), p-tyr-STAT-3 (Ray Biotech), mTOR (Ray Biotech) and HIF-1α (Ray Biotech) were conducted in the cell lysate …

Inhibition of 12/15 LOX ameliorates cognitive and cholinergic dysfunction in mouse model of hypobaric hypoxia via. attenuation of oxidative/nitrosative stress R Choudhary, U Malairaman, A Katyal - Neuroscience, 2017 - Elsevier … Hippocampal homogenate/plasma equivalent to 50-μg protein was added to the wells pre coated with monoclonal anti-mouse 12(S)HETE antibody (KinesisDX) and incubated for 1 h at 37 °C. This was followed by incubation with biotinylated anti-12HETE second antibody …

Salidroside exhibits anti-dengue virus activity by upregulating host innate immune factors N Sharma, KP Mishra, L Ganju - Archives of virology, 2016 - Springer … Supernatant was collected and kept at -80 °C. IFN-α and IRF-3 expression levels were quantified by ELISA (Blue Gene ELISA kit and Kinesis DX, Los Angeles, respectively) following the manufacturer's instructions. Reverse transcription polymerase chain reaction (RT-PCR) …

DADS analogues ameliorated the cognitive impairments of Alzheimer-like rat model induced by scopolamine A Manral, P Meena, V Saini, F Siraj, S Shalini… - Neurotoxicity …, 2016 - Springer … Enzyme-Linked Immunosorbent Assay (ELISA). The levels of Aβ42 were measured using double-antibody sandwich ELISA-based Aβ 1–42 assay kit. The assay was conducted following manufacturer's instructions (KinesisDX, USA) …

Biofunctionalized nanostructured zirconia for biomedical application: a smart approach for oral cancer detection S Kumar, S Kumar, S Tiwari, S Srivastava… - Advanced …, 2015 - Wiley Online Library By continuing to browse this site you agree to us using cookies as described in About Cookies. Remove maintenance message …

MicroRNA-145 Impedes Thrombus Formation via Targeting Tissue Factor in Venous Thrombosis A Sahu, PK Jha, A Prabhakar, HD Singh, N Gupta… - …, 2017 - ebiomedicine.com Non-invasive diagnosis of oral cancer: The role of electro-analytical methods and nanomaterials M Hasanzadeh, N Shadjou, M de la Guardia - TrAC Trends in Analytical …, 2017 - Elsevier

Novel insights into multitargeted potential of N′-(4-benzylpiperidin-1-yl) alkylamine derivatives in the management of Alzheimer's disease associated pathogenesis P Meena, A Manral, V Nemaysh, V Saini, F Siraj… - RSC Advances, 2016 - pubs.rsc.org

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